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lec-1 cell line  (BioWhittaker Molecular Applications)

 
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    BioWhittaker Molecular Applications lec-1 cell line
    Lec 1 Cell Line, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lec-1+cell+line/pm09214466-45-4-10?v=BioWhittaker+Molecular+Applications
    Average 90 stars, based on 1 article reviews
    lec-1 cell line - by Bioz Stars, 2026-08
    90/100 stars

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    (A) Comparison of binding of 125I-labeled porcine rotavirus to normal and glycosylation-defective cells. CHO-K1 cells, two lectin-resistant mutants <t>(Lec-1</t> and Lec-2) and their parental Pro-5 cells, MA-104 cells, and Caco-2 cells were harvested from tissue culture, suspended to a density of 2 × 106 per ml, and tested for their ability to bind 125I-rotavirus as described in Materials and Methods. The percentage bound at each time point divided by the surface area of the respective cell type is plotted versus the length of incubation. Symbols: ○, Lec-2 cells; ×, Caco-2 cells; ⧫, MA104 cells; ▴, Lec-1 cells; ■, CHO cells; •, Pro-5 cells. (B) Reconstitution of rotavirus binding to sialic acid-defective cells by exogenous addition of NeuGcGM3. Lec-2 cells were preincubated with purified porcine intestinal NeuGcGM3, rinsed, and challenged with 125I-rotavirus TLP as described in Materials and Methods. Virus bound per 106 NeuGcGM3-treated, sham-treated, and or CHO-K1 cells is shown.
    Lectin Resistant Mutant Cell Lines Lec 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lec-1+cell+line/pmc00110325-260-0-29?v=ATCC
    Average 93 stars, based on 1 article reviews
    lectin resistant mutant cell lines lec 1 - by Bioz Stars, 2026-08
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    mutant cell lines lec 1  (ATCC)
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    ATCC mutant cell lines lec 1
    (A) Comparison of binding of 125I-labeled porcine rotavirus to normal and glycosylation-defective cells. CHO-K1 cells, two lectin-resistant mutants <t>(Lec-1</t> and Lec-2) and their parental Pro-5 cells, MA-104 cells, and Caco-2 cells were harvested from tissue culture, suspended to a density of 2 × 106 per ml, and tested for their ability to bind 125I-rotavirus as described in Materials and Methods. The percentage bound at each time point divided by the surface area of the respective cell type is plotted versus the length of incubation. Symbols: ○, Lec-2 cells; ×, Caco-2 cells; ⧫, MA104 cells; ▴, Lec-1 cells; ■, CHO cells; •, Pro-5 cells. (B) Reconstitution of rotavirus binding to sialic acid-defective cells by exogenous addition of NeuGcGM3. Lec-2 cells were preincubated with purified porcine intestinal NeuGcGM3, rinsed, and challenged with 125I-rotavirus TLP as described in Materials and Methods. Virus bound per 106 NeuGcGM3-treated, sham-treated, and or CHO-K1 cells is shown.
    Mutant Cell Lines Lec 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lec-1+cell+line/pmc02140162-32-6-41?v=ATCC
    Average 93 stars, based on 1 article reviews
    mutant cell lines lec 1 - by Bioz Stars, 2026-08
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    lec-1 cell line  (BioWhittaker Molecular Applications)
    90
    BioWhittaker Molecular Applications lec-1 cell line
    (A) Comparison of binding of 125I-labeled porcine rotavirus to normal and glycosylation-defective cells. CHO-K1 cells, two lectin-resistant mutants <t>(Lec-1</t> and Lec-2) and their parental Pro-5 cells, MA-104 cells, and Caco-2 cells were harvested from tissue culture, suspended to a density of 2 × 106 per ml, and tested for their ability to bind 125I-rotavirus as described in Materials and Methods. The percentage bound at each time point divided by the surface area of the respective cell type is plotted versus the length of incubation. Symbols: ○, Lec-2 cells; ×, Caco-2 cells; ⧫, MA104 cells; ▴, Lec-1 cells; ■, CHO cells; •, Pro-5 cells. (B) Reconstitution of rotavirus binding to sialic acid-defective cells by exogenous addition of NeuGcGM3. Lec-2 cells were preincubated with purified porcine intestinal NeuGcGM3, rinsed, and challenged with 125I-rotavirus TLP as described in Materials and Methods. Virus bound per 106 NeuGcGM3-treated, sham-treated, and or CHO-K1 cells is shown.
    Lec 1 Cell Line, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lec-1+cell+line/pm09214466-45-4-10?v=BioWhittaker+Molecular+Applications
    Average 90 stars, based on 1 article reviews
    lec-1 cell line - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    (A) Comparison of binding of 125I-labeled porcine rotavirus to normal and glycosylation-defective cells. CHO-K1 cells, two lectin-resistant mutants (Lec-1 and Lec-2) and their parental Pro-5 cells, MA-104 cells, and Caco-2 cells were harvested from tissue culture, suspended to a density of 2 × 106 per ml, and tested for their ability to bind 125I-rotavirus as described in Materials and Methods. The percentage bound at each time point divided by the surface area of the respective cell type is plotted versus the length of incubation. Symbols: ○, Lec-2 cells; ×, Caco-2 cells; ⧫, MA104 cells; ▴, Lec-1 cells; ■, CHO cells; •, Pro-5 cells. (B) Reconstitution of rotavirus binding to sialic acid-defective cells by exogenous addition of NeuGcGM3. Lec-2 cells were preincubated with purified porcine intestinal NeuGcGM3, rinsed, and challenged with 125I-rotavirus TLP as described in Materials and Methods. Virus bound per 106 NeuGcGM3-treated, sham-treated, and or CHO-K1 cells is shown.

    Journal:

    Article Title: Structure and Function of a Ganglioside Receptor for Porcine Rotavirus

    doi:

    Figure Lengend Snippet: (A) Comparison of binding of 125I-labeled porcine rotavirus to normal and glycosylation-defective cells. CHO-K1 cells, two lectin-resistant mutants (Lec-1 and Lec-2) and their parental Pro-5 cells, MA-104 cells, and Caco-2 cells were harvested from tissue culture, suspended to a density of 2 × 106 per ml, and tested for their ability to bind 125I-rotavirus as described in Materials and Methods. The percentage bound at each time point divided by the surface area of the respective cell type is plotted versus the length of incubation. Symbols: ○, Lec-2 cells; ×, Caco-2 cells; ⧫, MA104 cells; ▴, Lec-1 cells; ■, CHO cells; •, Pro-5 cells. (B) Reconstitution of rotavirus binding to sialic acid-defective cells by exogenous addition of NeuGcGM3. Lec-2 cells were preincubated with purified porcine intestinal NeuGcGM3, rinsed, and challenged with 125I-rotavirus TLP as described in Materials and Methods. Virus bound per 106 NeuGcGM3-treated, sham-treated, and or CHO-K1 cells is shown.

    Article Snippet: Lectin-resistant mutant cell lines Lec-1 and Lec-2 and the parental Pro-5 CHO cells from which they were derived ( 3 , 29 – 31 ), all obtained from the ATCC (catalog no. crl 1735, crl 1736, and crl 1781, respectively), were grown in α-MEM supplemented with ribonucleosides, deoxyribonucleosides, and 10% FBS (Gibco; catalog no. 11900-024).

    Techniques: Binding Assay, Labeling, Incubation, Purification